Supporting Information Stereoselective hydroxylation of an achiral cyclopentanecarboxylic acid derivative using engineered P450s BM-3
نویسندگان
چکیده
a Institut für Organische Chemie der Technischen Universität Graz, Stremayrgasse 16, A-8010 Graz, Austria. Fax: +43 316 873 8740; Tel: +43 316 873 8751; E-mail: [email protected] b Division of Chemistry and Chemical Engineering, California Institute of Technology, Pasadena, CA 91125, USA. Fax: +1 626 568 8743; Tel: +1 626 395 4162; E-mail: [email protected] c Institut für Molekulare Biotechnologie der Technischen Universität Graz, Petersgasse 14, A-8010 Graz, Austria. Fax: 43 316 873 4071; Tel: 43 316 873 4074; E-mail: [email protected]
منابع مشابه
Stereoselective hydroxylation of an achiral cyclopentanecarboxylic acid derivative using engineered P450s BM-3.
Substrate engineered, achiral carboxylic acid derivative was biohydroxylated with various mutants of cytochrome P450 BM-3 to give two out of the four possible diastereoisomers in high de and ee. The BM-3 mutants exhibit up to 9200 total turnovers for hydroxylation of the engineered substrate, which without the protecting group is not transformed by this enzyme.
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